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Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo-transferrin

descriptionPublicationkeyboard_double_arrow_right Report , Other literature type 01 Jan 2020 Croatia Publisher:Zenodo
Authors: Štambuk, Tamara; Cvetko, Ana; Weitner, Tin;

doi: 10.5281/zenodo.4006017 , 10.5281/zenodo.4006018

Protocol for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo-transferrin

Abstract

Experimental protocol for for ultra performance liquid chromatography-mass spectrometry N-glycan analysis of the native and desialylated human apo-transferrin. Supplemental information for the published paper: Friganovi��, Tomislav; Toma��i��, Antonela; ��eba, Tino; Biru��, Ivan; Kerep, Robert; Borko, Valentina; ��aki��, Davor; Gabri��evi��, Mario; Weitner, Tin: Low-pressure chromatographic separation and UV/Vis spectrophotometric characterization of the native and desialylated human apo-transferrin // Heliyon, 7 (2021), 9; e08030, 8 doi:10.1016/j.heliyon.2021.e08030

This work was supported by funding from the Croatian Science Foundation grant UIP-2017- 05-9537 - Glycosylation as a factor in the iron transport mechanism of human serum transferrin (GlyMech), as well as the European Structural and Investments funding for the "Croatian National Centre of Research Excellence in Personalized Healthcare" (contract #KK.01.1.1.01.0010) and "Centre of Competences in Molecular Diagnostics" (contract #KK.01.2.2.03.0006).

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Croatia
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Keywords

UPLC, transferrin, N-glycan analysis, transferrin ; N-glycan analysis ; UPLC ; mass spectrometry, mass spectrometry

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