Publication . Other literature type . 2020

Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology

Name: Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology
Keywords: CRISPR, ddPCR, knockout, gene editing

Nicouleau, Michael; Pimentel, Luisa; Shlaifer, IrIna; Durcan, Thomas M.;

Open Access

English

doi: 10.5281/zenodo.3875777 , 10.5281/zenodo.3875776

Published: 04 Jun 2020

Publisher: Zenodo

- Summary

Abstract

This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes: CRISPR-Cas9 editing; Droplet digital PCR (ddPCR) technology; Single cell sorting.

Subjects

CRISPR, ddPCR, knockout, gene editing

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ZENODO

Other literature type . 2020

License: http://creativecommons.org/licenses/by/4.0/legalcode

Providers: Datacite