Aim: The aim of present study was to formulate and evaluate the Quinapril Hydrochloride loaded pulsatile microspheres by solvent evaporation technique. Method: Ethyl cellulose and Eudragit Rs 100 are biocompatible polymer is used as the retardant material. The effects of process conditions such as drug loading, polymer type and solvent type on the characteristics of microspheres were investigated. Results: The prepared microspheres were characterized for their particle size, drug loading and drug release. The in-vitro release studies were carried out in pH 1.2, phosphate buffer at pH 6.8 and pH 7.4. The prepared microspheres were white, free flowing and spherical in shape. The drug-loaded microspheres showed 89.30% of entrapment with particle size 235.2µm and the in-vitro release studies showed that Quinapril Hydrochloride microspheres of 85.14%. Conclusion: The study concluded that, the prepared microspheres formulation showed compliance with chronotherapeutic objective of hypertension.