This report describes the results obtained from the survey, interviews and interactions with associated partners and project stakeholders as part of the requirements analysis. The requirements analysis included surveys sent out to a large number of experts and designed to address issues relevant to: End users (e.g. members of academia, industry and regulatory agencies) Developers (tools developers, infrastructure provider and data managers Additionally, key persons were contacted for additional discussions to learn more about different infrastructure development or use in face-to-face and virtual interviews, at conferences like SOT, EuroTox and OpenTox as well as via webinars with question-and-answer sessions. The aim of the requirements analysis was to challenge the proposed concepts and to identify gaps in the existing approaches and unmet needs in the different communities. These were then used for improving and correcting the design concepts of the general infrastructure, the container orchestration approach, the API definition and the semantic interoperability layer and were translated into case studies. Requirements analyses performed in other projects (e.g. eNanoMapper, ToxBank) and interactions with ongoing projects (EU-ToxRisk, NanoCommons, projects of the NanoSafety Cluster) have been used to identify eventual elements already covered by other initiatives, which can be adopted, adapted if necessary and integrated into OpenRiskNet.