This article examines the diverse range of humor in the writings of one of America’s most renowned authors, Mark Twain. Through a variety of comedy forms, such as satire, irony, parody, wit, and burlesque, Twain’s humor is studied. The main themes and strategies that characterize Mark Twain’s humorous style are identified by this study through an analysis of selected works, including The Adventures of Huckleberry Finn, The Adventures of Tom Sawyer, A Connecticut Yankee in King Arthur’s Court. Twain’s use of humor to comment on the human condition, expose hypocrisy, and criticize social conventions is the main focus of the examination. This article concludes by arguing that Twain’s humor is a potent vehicle for moral reflection and social commentary in addition to being a source of amusement.