This report is a reflection on the impact of the work conducted by the SynergistEIC partners in supporting the startups from the 2nd cohort of the program. The main objective of this report is to assess and maximize the impact of the project activities, ensuring effective communication, engagement, and development. A project impact assessment framework was developed to closely monitor and understand how their engagement with the SynergistEIC project has helped the selected startups reach their objectives.The SynergistEIC project is an initiative funded under the European Union's Horizonprogram. It focuses on enhancing the European green technology (GreenTech) startup ecosystem in sectors like climate-tech, clean-tech, the circular economy, and agrifood. The project aligns with the EU's goals of boosting competitiveness and achieving Net Zero objectives. Within SynergistEIC, organizations from Romania (SPHERIK), Slovakia (G-FORCE), Portugal (FASTTRACK ACTION), and Ireland (F6S) aim to support GreenTech startups, helping them fine-tune their products and increase their market presence, and become more prepared to apply for and possibly win EIC Accelerator projects. SynergistEIC’s long-term strategic objective is to help European technology startups scale, contributing towards the competitiveness and strategic autonomy of the EU and fulfilling its ambitious Net Zero goals. The program does so by providing tailored, specific, and hands-on support to European impact-driven startups, in the GreenTech sector to become more competitive and increase their readiness to be successful in the EIC Accelerator. A special focus for the program is attracting high-potential companies from the Widening countries.