The cotton textile industry faces significant challenges due to the large amounts of contaminated effluent produced during cotton fiber processing,especially during desizing and pre-washing. To address this, new biotechnological tools are being developed to reduce water and chemical usage,minimizing pollution and using pollutants as feedstock to create valuable molecules. This work presents the production of a genetically modified bacterial carbohydrate oxidase, N-acetyl glucosamine oxidase (NagOX), to enhanceglucose acceptance as a substrate [1]. A key focus is the downstream process, where the enzyme is immobilized in a low-cost cellulosic matrix fromvarious sources via the fusion of a Carbohydrate Binding Module type 3 (CBM3) to the protein sequence (Figure 1) [2]. The immobilized enzyme canbe used in a continuous process to generate hydrogen peroxide from cotton textile process wastewater, which can then serve as a bleaching agentfor cotton goods.