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Western Blotting NSD3: Further Testing of Commercial Antibodies

descriptionPublicationkeyboard_double_arrow_right Report 09 Feb 2018Publisher:Zenodo

Authors: Dilworth, David; Barsyte-Lovejoy, Dalia;

doi: 10.5281/zenodo.1170474 , 10.5281/zenodo.1170473

Western Blotting NSD3: Further Testing of Commercial Antibodies

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Abstract

Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

SGC Open Notebook Project to Characterize the HMTase NSD3 Exp012 Objective: To test commercial antibodies to NSD3 by western blotting whole cell lysates from cancer cell lines, predominately of an AML background. Validated antibodies will be used as tools to study NSD3’s function on chromatin.

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