Habitat connectivity is a crucial biodiversity metric that illustrates human-induced challenges to ecosystems. To provide stakeholders with a decision-making tool for trade-offs related to land-use/land-cover (LULC) transformations, a workflow to compute terrestrial habitat connectivity was devised for the area of Catalonia (Spain), involving: (1) analysis of multi-sensor remote sensing imagery, (2) obtaining open-access spatial data on connectivity barriers, (3) LULC mapping, (4) content-analysis of connectivity studies, (5) computing connectivity using open-source Graphab software based on graph theory, (6) forecasting connectivity to reveal potential biodiversity loss triggered by LULC changes and (7) tools deployment through Docker containers and HPC mode.