Digital citizenship refers to the ability, confident and positive espousal with available digital technologies. It include such aspects like awareness, literacy, skills and is to achieve and understand digital literacy as well as to ensure online safety, cyber security, digital responsibility, digital health and well-being. This research study was quantitatively undertaken to explore the level of awareness and literacy on digital citizenship and harmonization of national database among university staffs in Nigeria. A scale was designed and adopted for this study. A set of 385 staffs were involved in the investigation. A Principal Component Analysis was affected upon the questionnaire items for validity with significance at 0.47, and also a reliability test was done where a significant Chronbach alpha value of 0.90 was established. The anticipated result from the analysis on the constructs indicated the followings: a moderate level of awareness and literacy on digital citizenship. It also indicated some significant level of perception on harmonization of relevant national databases. The result as well showed no statistical differences among the staff on level of awareness and literacy on digital citizenship between gender and between staff category; but there might be a statistical difference among them in terms of university.