This framework is the main output of a Knowledge Exchange (KE) study titled Approaches to scaling up reproducibility in research organisations. It will assist research institutions to understand how reproducibility can be scaled up internally. The framework focuses on meso-level factors, such as groups, organisations and communities, to understand the role of research organisations and the people within them (who both undertake and support research). The framework can be used by a range of internal stakeholders with differing goals, such as institutional leaders seeking to align organisational strategies, or managers wishing to provide the support that staff in their part of the organisation may be seeking. The intention is also to enable dialogue between managers and researchers to create collaborative and sustainable solutions for a wider uptake of reproducible research practices. The report, Approaches to scaling up reproducibility in research organisations, provides more detail about this work and can be accessed through this framework guidance document.