The present study was carried out to evaluate sensory and microbiological quality of buttermilk sold in and around Hyderabad city, India. The overall sensory score of buttermilk samples collected from organized, semi-organized and Hawkers was 8.42, 8.06 and 6.65 respectively under 10 point Headonic scale. The standard plate count were 3.52×104CFU/g, 8.56×104CFU/g, and 2.56×105 CFU/ml, the lactobacillus count were 2.48×104 CFU/ml, 6.52×104 CFU/ml and 8.95×104 CFU/ml, the coliforms count 2.51×101 CFU/ml, 3.52×102 CFU/ml, 8.58×102 CFU/ml and the yeast and mould were 3.58×102 CFU/ml, 6.58×102 CFU/ml and 1.52×103 CFU/ml for the samples from organized, Semi-organized and Hawkers respectively. The incidence of E.coli was 40%, 56% and 100%, Salmonella was 8%,12% and 36%, Staphylococcus 48%,60% and 100% and Campylobacter 8%,16% and 28% and counts of E.coli were 2.8CFU/ml, 8.5 CFU/ml and 32 CFU/ml, Salmonella were 4.2 CFU/ml, 6.5 CFU/ml and 8.8 CFU/ml and Staphylococcus were 8.58×102 CFU/ml, 2.86×103 CFU/ml and 3.52×104 CFU/ml and Campylobacter 1.2 CFU/ml, 2.8 CFU/ml and 4.8 CFU/ml for the samples from Organized, Semi-organized and Hawkers sectors respectively. The microbiological incidence and counts were high in Hawkers, least in Organized sector and in between in Semi-organized sector.