publication . Article . 2017

Analytical Method Development And Validation For The Simultaneous Estimation Of Sofosbuvir And Velpatasvir Drug Product By Rp-Hplc Method

Uppalapati.Jyothi*1, Dr.Parimi.Umadevi2;
Open Access
  • Published: 30 Aug 2017
  • Publisher: Zenodo
Abstract
A novel reversed phase high performance liquid chromatography (RP-HPLC) method has been developed for the estimation of Sofosbuvir and Velpatasvir drug product by liquid chromatography. The chromatographic separation was achieved on C18 column (XTerra RP18 150*4.6, 5um) at ambient temperature .The separation achieved employing a mobile phase consists of 0.1%v/v Trifluoro acetic acid in water: Methanol (42:58). The flow rate was 1.0 ml/ minute and ultra violet detector at 269nm. The average retention time for Sofosbuvir and Velpatasvir found to be 3.44 and 4.68 min. The proposed method was validated for selectivity, precision, linearity and accuracy. All validati...
Subjects
free text keywords: Sofosbuvir And Velpatasvir, Isocratic, HPLC, C18, Trifluoro Acetic Acid, Methanol And Validation., Sofosbuvir And Velpatasvir, Isocratic, HPLC, C18, Trifluoro Acetic Acid, Methanol And Validation.
Any information missing or wrong?Report an Issue