Background: Increased stress levels are associated with various health issues due to the dysregulation of HPA axis, more so when the stress is chronic in nature.As the DHEA-S is a catabolic hormone the chronic stress levels may be involved with long term diseases like early ageing, hypertension, cancer and various other diseases Objectives: The aim of this study was to see the effect of perceived chronic stress in a subset of healthy population of male and female volunteers who willingly participated in the study. The selected male and female population was than stratified into age and stress matched groups and then one time DHEA-S levels were ascertained to see for changes in the DHEA-S values. Methods: DHEA-S levels were measured within 6 hours of collection of the Blood Samples, randomly collected at different time points in the day by Chemiluminescence method which was than statistically evaluated by using Mini Tab statistical tool. Results: There was no significant correlation in both genders when age and stress matched.