This guide provides information and considerations for a fair and gender inclusive promotions and evaluations and gives practical advice to remove gender bias from the process aiming to ultimately raise the number of female scientists in top level positions. It was written as part of the work package Career Development in the framework of the project LIBRA - Leading Innovative measures to reach gender Balance in Research Activities, funded by Horizon2020 programme of the EU, and relies partly on the experience of 12 members of EU-LIFE, the Alliance of Research Institutes in the Life Sciences. Due its objective of achieving greater gender balance at the level of senior positions, the guide will focus on the transition from junior principal investigator (PI) to senior PI. A survey and director interviews conducted in 2018 at 12 EU-Life research institutes by the Task Force on PI Selection and Career Development collected the practices in the evaluation and promotion of faculty. They have helped to develop an exemplary evaluation process to serve as the basis for practical recommendations to reach gender inclusiveness. This exemplary process can be tailored to the individual situation.