Shampoo is a cosmetic preparation intended for cleansing the scalp and hair. However, frequent use of chemical-based formulations may lead to adverse effects such as hair fall, premature greying, and microbial resistance. To overcome these drawbacks, a polyherbal shampoo was formulated using hydro alcoholic extracts of Nimba, Karanja, Methika and infusion of Sathala, Japa, and Arishtaka for dandruff management. The study aimed to prepare, analyse, and evaluate the formulated polyherbal shampoo for its physicochemical, analytical, and antifungal efficacy. Phytochemical screening, FTIR, and HPTLC confirmed the presence of flavonoids, phenolics, saponins, and phytosterols. SEM analysis revealed smooth, intact hair cuticles after shampoo treatment, suggesting protective and conditioning effects. In vitro antifungal studies showed inhibition zones of 13 mm against Malassezia furfur and 15 mm against M. globosa. Thus, the formulated polyherbal shampoo demonstrated effective antifungal properties, supporting its use as a safe, natural alternative for dandruff management.