Citrus paradisi (Grapefruit) peel oil has medicinal properties which shows beneficial effects against plantpathogenic fungi Objective: The aim of this research was to investigate the antifungal potential ofCitrus paradisi peel oil against different plant pathogenic fungal strains Methods: The essential oil ofGrapefruit peel was provided in different concentrations 0.25 to 0.75ml per 100 ml of media to 3 differentplant pathogenic fungal strains i.e, Fusarium oxysporum, Fusarium solani and Dreschlera tetramera and itseffect on the growth of fungi was recorded Results: The studies provided the results compared with thecontrolled media, which showed the fungal growth was almost completely inhibited at 0.75mlconcentration of oil, even the lower concentration was sufficient to retard the growth Conclusions: Thestudy provided the blue print for fungicidal spray to cure serious diseases in plants, such as, rice blast,papaya fruit rot, seedling collar rot and wilt diseases.