Several species of the Lauraceae family are endangered, due to the high value of their woods, and the consequent exploitation of natural populations. Among them, Ocotea porosa, O. odorifera and O. catharinensis, native from South Brazil, have recalcitrant seeds, what makes even more dificult their natural regeneration. This work aimed to evaluate in vitro seed germination and multiplication of O. odorifera and O. catharinensis in culture medium supplemented with activated charcoal and/or BAP and in vitro seed germination and multiplication of O. porosa in culture medium supplemented with several concentrations of sucrose. The effect of BAP and activated charcoal were tested in the multiplication of O. catharinensis and O. odorifera and the effect of sucrose concentration (30, 60, 90 ou 120 g.L-1) was tested in the multiplication of O. porosa. The germination percentage was higher than 85% for all species. BAP promoted the highest multiplication rates for O. odorifera. O. porosa showed the best results for in vitro germination and multiplication on 60 g.L-1 sucrose. The establishment of O. catharinensis was achieved, but after some weeks, in all media tested, the explants showed abnormalities, and finally died.