publication . Other literature type . Doctoral thesis . 2017

A new approach to synthesis and application of novel classes of ligands for the affinity capture and characterisation of phosphorylated peptides

Agron, Mataj;
  • Published: 01 Jan 2017
  • Publisher: Monash University
Abstract
Mass spectrometry has emerged as the technique of choice for the detection of posttranslational modifications of proteins due to its capability in high-throughput, and high sensitive analyses of proteolytic ally derived peptides. The use of enrichment techniques in combination with mass spectrometry has led to the identification of numerous novel phosphorylated peptides from phosphorylated proteins from whole cell lysates. Despite recent advances in the field of mass spectrometry, the analyses of phosphorylated pep tides of very low abundance still poses major analytical challenges. This dissertation will present work that I have done to overcome the limitation ...
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