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Publication . Article . 2019

Alcian blue staining to track the intracellular fate of hyaluronic-acid-based nanoparticles at transmission electron microscopy

Flavia Carton; Mathieu Repellin; Giovanna Lollo; Manuela Malatesta;
Open Access
English
Published: 12 Dec 2019 Journal: European Journal of Histochemistry, volume 63, issue 4 (issn: 2038-8306, Copyright policy )
Publisher: PAGEPress Publications
Country: France
Abstract
The main step in the assessment of nanomaterial safety and suitability for biomedical use is the location and the dynamic tracking of nanoparticles (NPs) inside cells or tissues. To precisely investigate the uptake mechanisms and intracellular fate of NPs, transmission electron microscopy is the technique of choice; however, the detection of NPs may sometimes be problematic. In fact, while NPs containing strongly electron dense (e.g., metal) components do not require specific detection methods at the ultrastructural level, organic NPs are hardly detectable in the intracellular environment due to their intrinsic moderate electron density. In this study, the critical-electrolyte-concentration Alcian Blue method set up by Schofield et al. in 1975 was applied to track hyaluronic-acid-based NPs in muscle cells in vitro. This long-established histochemical method proved to be a powerful tool allowing to identify not only whole NPs while entering cells and moving into the cytoplasm, but also their remnants following lysosomal degradation and extrusion.
Subjects by Vocabulary

Library of Congress Subject Headings: lcsh:Biology (General) lcsh:QH301-705.5

Medical Subject Headings: technology, industry, and agriculture inorganic chemicals health care economics and organizations respiratory system

ACM Computing Classification System: ComputingMilieux_MISCELLANEOUS

Microsoft Academic Graph classification: Transmission electron microscopy Biophysics Microscopy Nanocarriers Ultrastructure Intracellular Nanoparticle Staining Nanomaterials Chemistry

Subjects

nanocarriers, hyaluronate, histochemistry, ultrastructure, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Cell Biology, Histology, Biophysics, Technical Note

41 references, page 1 of 5

1.Lim CT Han J Guck J Espinosa H.Micro and nanotechnology for biological and biomedical applications. Med Biol Eng Comput 2010;48:941-3. doi: 10.1007/s11517-010-0677-z 20844973 [PubMed]

2.Lollo G Rivera-Rodriguez GR Bejaud J Montier T Passirani C Benoit JP Polyglutamic acid-PEG nanocapsules as long circulating carriers for the delivery of docetaxel. Eur J Pharm Biopharm 2014;87:47-54. doi: 10.1016/j.ejpb.2014.02.004 24530693 [OpenAIRE] [PubMed]

3.Bobo D Robinson KJ Islam J Thurecht KJ Corrie SR Nanoparticlebased medicines: a review of FDAapproved materials and clinical trials to date. Pharm Res 2016;33:2373-87. doi: 10.1007/s11095-016-1958-5 27299311 [PubMed]

4.Fernandes RS Dos Santos Ferreira D de Aguiar Ferreira C Giammarile F Rubello D de Barros AL Development of imaging probes for bone cancer in animal models. A systematic review. Biomed Pharmacother 2016;83:1253-64. doi: 10.1016/j.biopha.2016.08.039 27565848 [PubMed]

5.Kaur P Aliru ML Chadha AS Asea A Krishnan S.Hyperthermia using nanoparticles—Promises and pitfalls. Int J Hyperthermia 2016;32:76-88. doi: 10.3109/02656736.2015.1120889 26757879 [OpenAIRE] [PubMed]

6.Sasso MS Lollo G Pitorre M Solito S Pinton L Valpione S Low dose gemcitabine-loaded lipid nanocapsules target monocytic myeloid-derived suppressor cells and potentiate cancer immunotherapy. Biomaterials 2016;96: 47-62. doi: 10.1016/j.biomaterials.2016.04.010 27135716 [OpenAIRE] [PubMed]

7.Asadi N Alizadeh E Salehi R Khalandi B Davaran S Akbarzadeh A.Nanocomposite hydrogels for cartilage tissue engineering: a review. Artif Cells Nanomed Biotechnol 2018;46:465-71. doi: 10.1080/21691401.2017.1345924 28743188 [PubMed]

8.Kavoosi F Modaresi F Sanaei M Rezaei Z.Medical and dental applications of nanomedicines. APMIS 2018;126:795-803. doi: 10.1111/apm.12890 30264432 [OpenAIRE] [PubMed]

9.Jahangirian H Lemraski EG Rafiee-Moghaddam R Webster TJ A review of using green chemistry methods for biomaterials in tissue engineering. Int J Nanomedicine 2018;13:5953-69. doi: 10.2147/IJN.S163399 30323585 [OpenAIRE] [PubMed]

10.Bamburowicz-Klimkowska M Poplawska M Grudzinski IP Nanocomposites as biomolecules delivery agents in nanomedicine. J Nanobiotechnology 2019;17:48. doi: 10.1186/s12951-019-0479-x 30943985 [OpenAIRE] [PubMed]

Funded by
EC| INVITE
Project
INVITE
INnovative Verona university’s Inter-disciplinary, Inter-sectoral and International Training Experience
  • Funder: European Commission (EC)
  • Project Code: 754345
  • Funding stream: H2020 | MSCA-COFUND-DP
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