Synthetic mRNA capping
Copyright policy )Synthetic mRNA capping
Eukaryotic mRNA with its 5′-cap is of central importance for the cell. Many studies involving mRNA require reliable preparation and modification of 5′-capped RNAs. Depending on the length of the desired capped RNA, chemical or enzymatic preparation – or a combination of both – can be advantageous. We review state-of-the art methods and give directions for choosing the appropriate approach. We also discuss the preparation and properties of mRNAs with non-natural caps providing novel features such as improved stability or enhanced translational efficiency.
Science, Q, enzymatic capping, Organic chemistry, Review, QD241-441, click chemistry, cap synthesis, RNA, methyltransferase, cap analogue
Science, Q, enzymatic capping, Organic chemistry, Review, QD241-441, click chemistry, cap synthesis, RNA, methyltransferase, cap analogue
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