The paper surveys the Quaternary (MN17–Q4) fossil record of Cricetinae in the Czech Republic and Slovakia with particular attention to the current glacial cycle (Q4: Vistulian–Holocene) and immediate history of extant taxa. Vast majority of records were reexamined using standardized techniques of morphometric analysis (in total 625 molars of Cricetus, 805 molars of small hamsters). Cricetus cricetus, recorded in 130 Q4 fossil communities, exhibits almost continuous appearance since the beginning of Vistulian, possibly with considerable range regressions during LGM and middle Holocene. It was recorded also in 27 Early Pleistocene (MN17–Q2) and 6 Middle Pleistocene (Q3) assemblages, with a small-sized form (Cricetus cricetus nanus) in MN17–Q1 stage and a mosaic of Q2–Q3 records exhibiting mostly excessively large size (C. c. runtonensis), similarly as the Vistulian items which are clearly larger than those of the post-LGM and early Holocene age. The appearance of hamster in fossil record shows a mosaic character suggesting temporal spatial and abundance fluctuations: in most sites it absents and in general, it appears as a rather rare sub-recedent element (in Q4 communities with overall dominance of mere 0.55% and this is valid also for its appearance in Early and Middle Pleistocene assemblages). Small hamsters arranged here in the genus Cricetulus (incl. Allocricetus) represent a constant element of the Early and Middle Pleistocene assemblages (43 sites in the Czech Republic and Slovakia). The items of Q4 record (21 communities) clearly differ from the Early to Middle Pleistocene form Cricetulus bursae by a consistently smaller size corresponding to extant species Cricetulus migratorius. The gap is quite distinct conforming even to the possible extinction of C. bursae during the Eemian interglacial followed by early Vistulian expansion of the extant species from its Asiatic range suggested by molecular phylogeography. We examined a question of possible appearance of Phodopus among the Q4 record of small hamsters, yet in our material, we did not succeed to confirm it.