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Sinop Üniversitesi Fen Bilimleri Dergisi
Article . 2023 . Peer-reviewed
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Hakkâri İlinde Üretilen Kabakgillerde Hıyar Mozaik Virüsü’nün DAS-ELISA Yöntemi ile Tespiti

Authors: Mehmet AKTAŞ; Nevin AKDURA;

Hakkâri İlinde Üretilen Kabakgillerde Hıyar Mozaik Virüsü’nün DAS-ELISA Yöntemi ile Tespiti

Abstract

Bu çalışmada; Hakkâri ilinde yetiştirilen kabakgil çeşitlerinde zararlı ve ekonomik kayıplara neden olan viral etmenlerden Hıyar mozaik virüsü (Cucumber mosaic virus; CMV), DAS-ELISA (Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay) yöntemi ile serolojik olarak araştırılmıştır. Bu viral etmenin dünya genelinde ciddi ekonomik kayıplara sebep olduğu yapılan çalışmalar ile bildirilmiştir. Bu çalışmada kullanılan yaprak örnekleri, Hakkâri ilinde kabakgil üretiminin yapıldığı Kırıkdağ, Otluca ve Merzan alanlarından virüs hastalığı açısından şüpheli bulunan yapraklarda sararma, nekroz, mozaik ve şekil bozukluğu semptomları sergileyen kabakgillerden toplanmıştır. Viral belirti gösteren 7 adet karpuz (Citrullus lanatus L.), 5 adet kavun (Cucumis melo L.), 38 adet hıyar (Cucumis sativus L.), 15 adet kabak (Cucurbita sp.), 20 adet bal kabağı (Cucurbita pepo) ve 7 adet acur (Cucumis melo var. flexuosus) olmak üzere toplam 92 adet yaprak örneği Eylül 2022’de toplanmış ve örneklere CMV enfeksiyonunun tanılanması amacı ile DAS-ELISA testi uygulanmıştır. DAS-ELISA testi ile 92 örneğin 29’unda CMV tespit edilmiştir. CMV enfekteli örnek sayısı ile en fazla %31.52 oranında Otluca’da tespit edilmiştir. Kabakgil çeşitlerinde ise test edilen 7 örneğin 6’sında %85.71 ile acurda tespit edilmiştir.

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Keywords

Biochemistry and Cell Biology (Other), Cucurbit;CMV;DAS-ELISA;Hakkâri, Biyokimya ve Hücre Biyolojisi (Diğer), Kabakgil;CMV;DAS-ELISA;Hakkâri

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    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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