publication . Article . 2020

HPLC-MS/MS Method for Quantitation of Sildenafil and Its Active Metabolite in Human Plasma

Open Access
  • Published: 01 Sep 2020 Journal: The Bulletin of the Scientific Centre for Expert Evaluation of Medicinal Products, volume 10, pages 192-200 (issn: 1991-2919, eissn: 2619-1172, Copyright policy)
  • Publisher: SCEEMP
A high demand for sildenafil-based drugs puts a premium on the development of methods for quantitation of sildenafil in bio-substrates to facilitate pharmacokinetic analysis in bioequivalence studies. High performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was proposed for the method development due to its high sensitivity, selectivity, reproducibility, and performance. The aim of the study was to develop and validate an HPLC-MS/MS method for quantitative determination of sildenafil and its active metabolite N-desmethyl sildenafil in human plasma. Approbation of the developed technique in the study of the pharmacokinetic profile...
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Medical Subject Headings: respiratory tract diseasescardiovascular system
free text keywords: validation, n-desmethyl sildenafil, hplc-ms/ms, Chromatography, Reproducibility, High-performance liquid chromatography, Active metabolite, Vardenafil, medicine.drug, medicine, Triple quadrupole mass spectrometer, Bioequivalence, Sildenafil, chemistry.chemical_compound, chemistry, Pharmacokinetics, business.industry, business, lcsh:Medicine (General), lcsh:R5-920
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