publication . Article . 2019

STUDY OF DNA TRANSFORMATION DYNAMICS IN ВНК-21/2-17 CELL CULTURE USING FLOW CYTOMETRY DURING FMD VIRUS REPRODUCTION

M. N. Guseva; M. A. Shevchenko; D. V. Mikhalishin; V. V. Mikhalishin; B. L. Manin;
Open Access
  • Published: 01 Jan 2019 Journal: Veterinary Science Today (issn: 2304-196X, Copyright policy)
  • Publisher: FGI Federal Centre for Animal Health (FGI ARRIA)
Abstract
<jats:p>The research tasks covered the study of ВНК-21/2-17 cell DNA transformation dynamics during FMDV reproduction process. It was noted that the destruction of major cell population coincided with the increase in apoptotic cell number and detritus amount. Three hours post cell culture infection increase in apoptosis and detritus was observed, G 1-phase decreased by 17–21% and polynuclear cells grew by 2.3 times. In seven hours, the drastic rise in cell death was noted. It was established that at all stages of FMDV culture in ВНК-21/2-17 suspension cell line, diploid cells G1(2n) were predominant, being basic cells for the virus reproduction. Cells in synthet...
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free text keywords: flow cytometry, cell cycle phases, внк-21/2-17 cell suspension, foot-and-mouth disease virus, Biology, Transformation (genetics), Fmd virus, Flow cytometry, medicine.diagnostic_test, medicine, Cell culture, Virology, lcsh:Veterinary medicine, lcsh:SF600-1100
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