The present investigation was carried out to study variability induced by gamma rays with respect to vegetative characters and LD50 dose in Rough lemon. Rough lemon seeds were gamma irradiated at doses of 0, 4, 6 and 8 kr along with control. Seed germination decreased with increasing dose of gamma radiation. Seedling height and leaf size also decreased with increasing dose of gamma radiation, whereas, apical branching, number of branches/seedling, number of variegated / albino seedlings and number of leaves increased with increasing dose of gamma radiation. Maximum variability for seedling height, number of leaves, leaf size, colour, internode length, and per cent apical branching was observed at two months from sowing in seeds treated with 8 kr dose of gamma radiation. Variability for all characters was, however, found to be minimum in the control.