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Journal of Oral Science
Article . 2008 . Peer-reviewed
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Journal of Oral Science
Article
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Journal of Oral Science
Article . 2009
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https://dx.doi.org/10.2334/jos...
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In vitro evaluation of the cytotoxicity of ProRoot MTA and MTA Angelus

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2008 English Publisher:Nihon University School of DentistryJournal:Journal of Oral Science, volume 50, pages 397-402 (issn: 1343-4934, eissn: 1880-4926, Copyright policy )
Authors: Elisabeth A, Koulaouzidou; Nikolaos, Economides; Panagiotis, Beltes; George, Geromichalos; Konstantinos, Papazisis;

doi: 10.2334/josnusd.50.397

pmid: 19106466

In vitro evaluation of the cytotoxicity of ProRoot MTA and MTA Angelus

Abstract

The purpose of the present in vitro study was to compare the cytotoxic effect of two commercially available brands of mineral trioxide cement (ProRoot MTA and MTA Angelus), modified zinc oxide-eugenol cement (SuperEBA) and resin-modified glass ionomer cement (Vitrebond) using rat pulp cells (RPC-C2A) and human lung fibroblasts (MRC-5). The cells were cultured in typical culture conditions and exposed to the tested materials by adaptation of insert wells. The cytotoxic effect was recorded at two observation periods (24 and 72 h) by using a colorimetric assay of tetrazolium reduction (XTT method) in reference to controls. Overall, the degree of cytotoxic effect in ascending order was ProRoot MTA - MTA Angelus < SuperEBA < Vitrebond. Both MTA materials tested exerted mild suppression of cellular mitochondrial activity and may be characterized as biologically inert materials.

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Keywords

Cell Survival, Cell Culture Techniques, Biocompatible Materials, Cell Count, Oxides, Calcium Compounds, Fibroblasts, Cell Line, Mitochondria, Drug Combinations, Glass Ionomer Cements, Dentin-Bonding Agents, Materials Testing, Animals, Humans, Colorimetry, Indicators and Reagents, Aluminum Compounds, Lung, Dental Pulp

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    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
48
Top 10%
Top 10%
Top 10%
gold