Alfalfa is one of the most significant forage crops in Serbia, and is known colloquially as ?the queen of forage crops? due its significant role in livestock nutrition. Production of high quality forage and seed is of high importance for animal husbandry in Serbia. Fungi infections decrease seed quality which has influence on yield and quality of forage and on period of alfalfa utilization. Samples of alfalfa seed from Middle Banat, Danube region, Nis and Sumadija area were examined. Samples were taken from two alfalfa cultivars K-22 and K-28. Based on the examinations of appearance of fungi on the alfalfa seed, following fungi were determined: Fusarium spp., Alternaria spp., Stemphylium spp., Epiccocum spp., Nigrospora spp., Rhizopus spp., Mucor spp., Aspergillus spp. and Penicillium spp. Primary species were from genera Fusarium and Alternaria, and other species were represented in different percentages.