Powered by OpenAIRE graph
Found an issue? Give us feedback
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ DOAJarrow_drop_down
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
DOAJ
Article . 2014
Data sources: DOAJ
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
Hemijska Industrija
Article . 2014
Data sources: DOAJ-Articles
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
Hemijska Industrija
Article
License: CC BY NC ND
Data sources: UnpayWall
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
Hemijska Industrija
Article . 2014 . Peer-reviewed
License: CC BY
Data sources: Crossref
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
versions View all 3 versions
addClaim

This Research product is the result of merged Research products in OpenAIRE.

You have already added 0 works in your ORCID record related to the merged Research product.

Immobilization of chemically modified horse radish peroxidase within activated alginate beads

Authors: Dragica Spasojević; Miloš Prokopijević; Olivera Prodanović; Marilen Pirtea; Ksenija Radotić; Radivoje Prodanovic;

Immobilization of chemically modified horse radish peroxidase within activated alginate beads

Abstract

Immobilization of horse radish peroxidase (HRP) within alginate beads was improved by chemical modification of the enzyme and polysaccharide chains. HRP and alginate were oxidized by periodate and subsequently modified with ethylenediamine. Highest specific activity of 0.43 U/ml of gel and 81 % of bound enzyme activity was obtained using aminated HRP and alginate oxidized by periodate. Immobilized enzyme retained 75 % of original activity after 2 days of incubation in 80 % (v/v) dioxane and had increased activity at basic pH values compared to native enzyme. During repeated use in batch reactor for pyrogallol oxidation immobilized peroxidase retained 75 % of original activity. [Projekat Ministarstva nauke Republike Srbije, br. ON173017 i br. ON172049]

Subjects by Vocabulary

Microsoft Academic Graph classification: Immobilized enzyme Horseradish peroxidase chemistry.chemical_compound Chromatography biology Chemistry Chemical modification Periodate Enzyme assay Pyrogallol biology.protein Specific activity Peroxidase

Library of Congress Subject Headings: lcsh:Chemical technology lcsh:TP1-1185

Keywords

General Chemical Engineering, peroxidase, TP1-1185, Chemical technology, immobilized, General Chemistry, periodate, ethylene diamine

14 references, page 1 of 2

41 (2011) 770-804. [3] G.V. Presnova, M.Y. Rybcova, A.M. Egorov, Electroche-

J. Gen. Chem. 78 (2008) 2482-2488. [4] D. Brady, J. Jordaan, Advances in enzyme immobil-

isation, Biotechnol. Lett. 31 (2009) 1639-1650. [5] J.K. Park, H.N. Chang, Microencapsulation of microbial

cells, Biotechnol. Adv. 18 (2000) 303-319. [6] S.V. Mohan, K.K. Prasad, N.C. Rao, P.N. Sarma, Acid azo

peroxidase (HRP) catalyzed process, Chemosphere 58

(2005) 1097-1105. [7] Y.J. Hong, M.S. Lee, J.C. Kim, pH-dependent release of

17 (2011) 410-414. [8] R.C. Rodrigues, A. Berenguer-Murcia, R. Fernandez-Lafu-

mes, Adv. Synth. Catal. 353 (2011) 2216-2238. [9] U.J. Trivedi, A.S. Bassi, J. Zhu, Investigation of phenol

seed hull peroxidase, Can. J. Chem. Eng. 84 (2006) 239-

-247. [10] F. Quintanilla-Guerrero, M.A. Duarte-Vazquez, B.E. Gar-

  • BIP!
    Impact byBIP!
    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    7
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
    OpenAIRE UsageCounts
    Usage byUsageCounts
    visibility views 206
    download downloads 412
  • citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    7
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
    Powered byBIP!BIP!
  • 206
    views
    412
    downloads
    Powered byOpenAIRE UsageCounts
Powered by OpenAIRE graph
Found an issue? Give us feedback
visibility
download
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
OpenAIRE UsageCountsViews provided by UsageCounts
downloads
OpenAIRE UsageCountsDownloads provided by UsageCounts
7
Average
Average
Average
206
412
gold