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Hemijska Industrija
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Characterization of amyloglucosidase immobilized on the copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate in simulated industrial conditions

Authors: Nenad Milosavić; Radivoje Prodanovic; Slobodan M. Jovanović; Vuk M. Maksimović; Zoran Vujčić;

Characterization of amyloglucosidase immobilized on the copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate in simulated industrial conditions

Abstract

The application of amyloglucosidase immobilized on the macroporous co-polymer of ethylene glycol dimethacrylate and glycidyl methacrylate (poly (GMA-co-EGDMA)) in an enzyme reactor was shown. The higher thermostability of immobilized glucoamylases than the soluble one was demonstrated. Immobilized amyloglucosidase obtained by the periodate method shows two times higher thermo stability than the soluble form. Glucoamylases immobilized on poly (GMA-co-EGDMA) have good mechanical and chemical features in the reactor and when applied in a continuous flow reactor for 28 days no changes are observed. In this period periodate immobilized amyloglucosidase shows no decrease in activity. It showed potential for the continuous production of glucose from starch over a prolonged period of time.

Subjects by Vocabulary

Microsoft Academic Graph classification: Glycidyl methacrylate Starch Ethylene glycol dimethacrylate chemistry.chemical_compound Copolymer Amylase Thermostability Chromatography biology Periodate chemistry Enzyme reactor biology.protein Nuclear chemistry

Library of Congress Subject Headings: lcsh:Chemical technology lcsh:TP1-1185

Keywords

General Chemical Engineering, glucoamylase, TP1-1185, Chemical technology, General Chemistry, stability, periodate, poly (GMA-co-EGDMA), immobilization, enzyme reactor

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
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