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Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae

Authors: Ratko Pavlović; Marica Grujic; Biljana Dojnov; Miroslava Vujčić; Vera Nenadovic; J. Ivanović; Zoran Vujčić;

Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae

Abstract

The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven ?-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and ?-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-?-1,4-glucanase; subsequently, the obtained fragments are broken down by ?-D-glucosidase mostly in middle midgut.

Country
Serbia
Subjects by Vocabulary

Microsoft Academic Graph classification: Cellulase Native page Biology Nutrient Botany Cerambyx cerdo Zymography Larva Midgut biology.organism_classification biology.protein Longhorn beetle

Library of Congress Subject Headings: lcsh:QH301-705.5 lcsh:Biology (General)

Keywords

QH301-705.5, midgut, Cerambyx cerdo, General Biochemistry, Genetics and Molecular Biology, β-D-glucosidase, Cerambycidae, Biology (General), beta-D-glucosidase, zymogram, cellulase, isoforms, endocellulase, General Agricultural and Biological Sciences, Serbia

39 references, page 1 of 4

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Božić N., Vujčić Z., Janković-Tomanić M., Ivanović J., and V. Nenadović (2001). Proteinazne aktivnosti srednjeg creva larve Cerambyx cerdo (Coleoptera, Cerambycidae). Acta entomologica Serbica, 6, 1-2, 143-146.

Božić, N., Vujčić, Z., Nenadović, V., and J. Ivanović (2003). Partial puricfiation and characterization of midgut leucyl aminopeptidase of Morimus funereus (Coleoptera: Cerambycidae) larvae. Comp. Biochem. Physiol. 134B, 231-234. [OpenAIRE]

Božić, N., Vujčić, Z., Nenadović, V., and J. Ivanović (2004). Isoforms of leucyl - aminopeptidase of Cerambyx cerdo (Coleopterae, Cerambycidae) larvae, Arch. Biol. Sci., 56(1-2), 1P-2P. [OpenAIRE]

Bradford, M.M., (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.

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Chararas, C., Eberhard, R., Courtois, J. E., and F. Petek (1983). Purification of three cellulases from the xylophagous larvae of Ergates faber (Coleoptera: Cerambycidae). Insect Biochemistry 13 (2), Pages 213-218.

Council of Europe (1996) Background information on invertebrates of the Habitats Directive and the Bern Convention, Nature and environment, No 79, Council of Europe Publishing, Strasbourg, 59-62

Cristofoletti, P., Ribeiro, A. and W. Terra (2001). Apocrine secretion of amylase and exocytosis of trypsin along the midgut of Tenebrio molitor larvae. J. Insect Physiol., 47, 143-155.

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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views
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