Acute hypoxia induces upregulation of microRNA-210 expression in glioblastoma spheroids
Copyright policy )Acute hypoxia induces upregulation of microRNA-210 expression in glioblastoma spheroids
Tumor hypoxia and presence of tumor stem cells are related to therapeutic resistance and tumorigenicity in glioblastomas. The aim of the present study was therefore to identify microRNAs deregulated in acute hypoxia and to identify possible associated changes in stem cell markers.Glioblastoma spheroid cultures were grown in either 2 or 21% oxygen. Subsequently, miRNA profiling was performed and expression of ten stem cell markers was examined.MiRNA-210 was significantly upregulated in hypoxia in patient-derived spheroids. The stem cell markers displayed a complex regulatory pattern.MiRNA-210 appears to be upregulated in hypoxia in immature glioblastoma cells. This miRNA may represent a therapeutic target although it is not clear from the results whether this miRNA may be related to specific cancer stem cell functions.
- Odense University Hospital Denmark
- University of Southern Denmark Denmark
Time Factors, Gene Expression Profiling, Hypoxia-Inducible Factor 1, alpha Subunit, Cell Hypoxia, Up-Regulation, MicroRNAs, Cell Line, Tumor, Spheroids, Cellular, Neoplastic Stem Cells, Humans, Glioblastoma, Oligonucleotide Array Sequence Analysis
Time Factors, Gene Expression Profiling, Hypoxia-Inducible Factor 1, alpha Subunit, Cell Hypoxia, Up-Regulation, MicroRNAs, Cell Line, Tumor, Spheroids, Cellular, Neoplastic Stem Cells, Humans, Glioblastoma, Oligonucleotide Array Sequence Analysis
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