publication . Other literature type . Thesis . 2016 . Embargo end date: 02 Jan 2018

A NOVEL PIPELINE FOR DRUG DISCOVERY IN NEUROPSYCHIATRIC DISORDERS USING HIGH-CONTENT SINGLE-CELL SCREENING OF SIGNALLING NETWORK RESPONSES EX VIVO

Lago Cooke, Santiago Guillermo;
English
  • Published: 07 Oct 2016
  • Publisher: Apollo - University of Cambridge Repository
  • Country: United Kingdom
Abstract
The current work entails the development of a novel high content platform for the measurement of kinetic ligand responses across cell signalling networks at the single-cell level in distinct PBMC subtypes ex vivo. Using automated sample preparation, fluorescent cellular barcoding and flow cytometry the platform is capable of detecting 21, 840 parallel cell signalling responses in each PBMC sample. We apply this platform to characterize the effects of neuropsychiatric treatments and CNS ligands on the T cell signalling repertoire. We apply it to define cell signalling network abnormalities in PBMCs from drug-naïve first-onset schizophrenia patients (n=12) relativ...
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free text keywords: drug discovery, neuropsychiatric disorders, high-content screening, single-cell, signalling network
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