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Czech Journal of Animal Science
Article . 2009 . Peer-reviewed
License: CC BY NC
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Czech Journal of Animal Science
Article
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Czech Journal of Animal Science
Article . 2009
Data sources: DOAJ
https://dx.doi.org/10.17221/17...
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Analysis of rabbit cell surface (CD) antigens by means of cross-reactive monoclonal antibodies with specificity for cattle CD antigens

descriptionPublicationkeyboard_double_arrow_right Article 30 Jun 2009Publisher:Czech Academy of Agricultural SciencesJournal:Czech Journal of Animal Science, volume 54, pages 270-276 (issn: 1212-1819, eissn: 1805-9309, Copyright policy )
Authors: M. Simon; J. Antalíková; Ľ. Horovská; J. Jankovičová; K. Fábryová; S. Hluchý; P. Chrenek; +1 Authors

doi: 10.17221/1728-cjas

Analysis of rabbit cell surface (CD) antigens by means of cross-reactive monoclonal antibodies with specificity for cattle CD antigens

Abstract

Studies that involved testing monoclonal antibodies (mAbs) for cross-species reactivity proved to be efficient for the identification of previously unrecognized antigens in a number of different species. Twenty-six mAbs specific to different bovine CD (cluster defined) antigens (CD9, CD18, CD45R, CD41/61, CD62L, MHC class I and bovine IgG light chain molecule) were assayed for reactivity with rabbit peripheral blood leukocytes. Four of the mAbs recognizing CD9 and CD41/61 were reactive with rabbit platelets or granulocytes. These were investigated further by immunoblotting and immunohistochemical staining. The study identified CD9 and CD41/61 molecules on rabbit cells by mAbs IVA-50 and IVA-38. It showed that IVA-50 is a new valuable CD9 reagent for rabbit immunology which could be used for immunofluorescence staining or ELISA assay, immunohistological and molecular studies of rabbit CD9 antigen. IVA-38 recognizes the CD41/61 on rabbit platelets in indirect immunofluorescence and ELISA assay.

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Keywords

cd41/61, tissue, cd9, blood cells, SF1-1100, Animal culture

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
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