publication . Article . 2017

Application of tissue engineering technology for formation of human articular cartilage in perfusion bioreactor

V. I. Sevastianov; Yu. B. Basok; A. M. Grigoriev; L. A. Kirsanova; V. N. Vasilets;
Open Access Russian
  • Published: 01 Sep 2017 Journal: Vestnik Transplantologii i Iskusstvennyh Organov, volume 19, issue 3, pages 81-92 (issn: 1995-1191, Copyright policy)
  • Publisher: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
Abstract
<jats:p>Formation of tissue-engineered construct was performed in a specially developed bioreactor. At first, a cellengineered construct of human cartilage tissue consisting of biopolymer microstructured collagen-containing hydrogel, mesenchymal stromal cells of human adipose tissue (hADMSCs) and induction chondrogenic culture medium was prepared and placed in a perfusion bioreactor. As a result, on the 16th day of the study hADMSCs obtain a flattened shape typical for chondroblasts and demonstrate high proliferative activity with the formation of their own extracellular matrix. Histological analysis of the cultured system indicates the beginning of the formatio...
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Subjects
free text keywords: perfusion bioreactor, cell-engineered construct of human articular tissue, tissue-engineered construct of human articular tissue, biopolymer matrix, mesenchymal stromal cells, cartilage tissue, Immunology and Allergy, Transplantation, lcsh:Surgery, lcsh:RD1-811, Chemistry, Articular cartilage, Tissue engineering, Biomedical engineering
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