We present an annotated list of anuran species found in the Parque Municipal de Piraputangas, a protected area of a semi-deciduous forest located in Corumbá, Mato Grosso do Sul, Brazil. Fieldwork was conducted three times per month between August 2012 and July 2013. We recorded 29 species from six families (Bufonidae, Dendrobatidae, Hylidae, Leptodactylidae, Microhylidae, and Phyllomedusidae), representing 30% of the anuran species known to occur in Mato Grosso do Sul and 50% of the anurans recorded in this state’s semi-deciduous forests. Microhylids, leptodactylids, and bufonids were associated with terrestrial substrates, whereas most hylids and phyllomedusids were found perched on vegetation. This study will fill gaps in the knowledge on the anuran fauna of the western border of the Pantanal and will serve as a basis for additional studies that are needed to understand how species respond to habitat loss or fragmentation, such as landscape conversions, commercial logging, or mining.