Proteins can be procured from both plants and animals. Plant proteins are more preferable as the animal proteins may cause adverse health effects to human life. Proteins derived from plant sources are less expensive and hence are more cost effective. Quality of proteins relies on several factors and biological value is one such factor. Proteins with major essential amino acid are with high biological value. Every plant source is deficient in one or more essential amino acids so it is recommended to include multiple plant-based diets. Also proteins obtained from plant sources are less palatable so it is important to add flavor in order to make it more palatable. The quality and quantity of the proteins also depend on the techniques used for isolation and purification of proteins. Elucidation of the structure of proteins involves the use of techniques like nuclear magnetic resonance, X-ray crystallography and spectroscopy. Apart from the structural analysis the functioning of the protein could be determined by amino acid sequencing which could be performed using mass spectroscopy. Ultrasound assisted extraction, enzyme assisted protein extraction and electro activation method are few of the isolation and purification method which can be used in isolation and purification of these proteins. Owing to the vast availability of plant-based proteins it has various industrial applications like, plant based protein can be used as a dairy substitute, plant based meat analogue and its use as bioactive peptides which have been briefly discussed in the review.