Introduction. An Introduction to Plant Cell Culture: Pointers to Success R. D. Hall. Cell Culture and Plant Regeneration. Callus Initiation Maintenance and Shoot Induction in Rice N. W. Blackhall J. P. Jotham K. Azakanandam J. B. Power K. C. Lowe E. C. Cocking and M. R. Davey. Callus Initiation Maintenance and Shoot Induction in Potato:Monitoring of Spontaneous Genetic Variability In Vitro and In Vivo R. F. Curry and A. C. Cassells. Somatic Embryogenesis in Barley Suspension Cultures M. Kihara H. Funatsuki K. Ito and P. A. Lazzeri. Somatic Embryogenesis in Picea Suspension Cultures U. Egertsdotter. Specialized Techniques. Direct Cyclic Somatic Embryogenesis in Cassava for Mass Production Purposes K. J. J. M. Raemakers E. Jacobsen and R. G. F. Visser. Immature Inflorescence Culture of Cereals S. Rasco-Gaunt and P. Barcelo. Cryopreservation of Rice Tissue Cultures E. E. Benson and P. T. Lynch. Non-Cryogenic Long-Term Germplasm Storage of Potato A. Golmirzaie and J. Toledo. Plant Propagation In Vitro. Microprogation of Strawberry via Axillary Shoot Proliferation P. Boxus. Meristem-Tip Culture for Propagation and Virus Elimination B. W. W. Grout. Specialized Techniques. Clonal Propagation of Orchids B. Tisserat and D. Jones. Clonal Propagation of Cacti J. Gratton and M. F. Fay. Microproragation of Flower Bulbs: Lily and Narcissus M. M. Langens-Gerrits and G-J . M. de Klerk. Clonal Propagation of Conifers I. S. Harry and T. A. Thorpe. The Culture of Ferns M. V. Ford and M. F. Fay. Applications for Plant Protoplasts. Protoplast Isolation Culture and Plant Regeneration from Passiflora Leaves P. Anthony W. Otoni J. B. Power K. C. Lowe and M. R. Davey. Isolation Culture and Plant Regeneration from Suspension-Derived Protoplasts of Lolium M. Folling and A. Olesen. Protoplast Fusion for Symmetric Somatic Hybrid Production in Brassicaceae J. Fahleson and K. Glimelius. Production of Cybrids in Rapeseed (Brassica napus) S. Yarrow. Specialized Techniques. Microprotoplast Isolation and the Production of Monosomic Addition Lines K. S. Ramulu. Guard Cell Protoplasts: Isolation Culture and Regeneration of Plants G. Boorse and G. Tallman. In Vitro Fertilization with Isolated Single Gametes E. Kranz. Protocols for Genomic Manipulation. Anther and Microspore Culture in Barley H. Lorz and A. J"hne-G"rtner. Microspore Embryogenesis and In Vitro Pollen Maturation for the Production of (Double) Haploids in Tobacco E. Heberle-Bors. Embryo Rescue Following Wide Crosses H. C. Sharma. Specialized Techniques. Mutagenesis and the Selection of Resistant Mutants P. J. Dix. The Generation of Plastid Mutants In Vitro P. J. Dix. Protocols for the Introduction of Specific Genes. Agrobacterium-Mediated Transformation of Petunia Leaf Discs I. M. van der Meer. Transformation of Rice Via PEG-Mediated DNA Uptake into Protoplasts K. Datta and S. K. Datta. Transformation of Wheat Via Particle Bombardment I. K. Vasil and V. Vasil. Plant Transformation via Protoplast Electroporation G. W. Bates. Specialized Techniques. Transformation of Maize via Tissue Electroporation K. D'Halluin E. Bonne M. Bossut and R. Le Page. Transformation of Maize Using Silicon Carbide Whiskers J. M. Dunwell. Suspension Culture Initiation and the Accumulation of Metabolites. Directing Anthraquinone Accumulation via Manipulation of Morinda Suspension Cultures M. J. M. Hagendoorn D. C. L. Jamar and L. H. W. van der Plas. Alkaloid Accumulation in Catharanthus roseus Suspension Cultures A. H. Scragg. Betalains: Their Accumulation and Release In Vitro C.S. Hunter and N. J. Kilby. Appendix. Index.