cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR Kris N. Lambert and Valerie M. Williamson Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations David R. Sibson and Michael P. Starkey Isolation of Messenger RNA from Plant Tissues Alison Dunn cDNA Library Construction for the Lambda ZAP(R)-Based Vectors Marjory A. Snead, Michelle A. Alting-Mees, and Jay M. Short Clone Excision Methods for the Lambda ZAP(R)-Based Vectors Marjory A. Snead, Michelle A. Alting-Mees, and Jay M. Short Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs Yue Zhang and Michael A. Frohman Inverse PCR Approach to Cloning cDNA Ends Sheng-He Huang Cloning Gene Family Members Using the Polymerase Chain Reaction with Degenerate Oligonucleotide Primers Gregory M. Preston Subtractive Hybridization for the Isolation of Differentially Expressed Genes Using Magnetic Beads Hans-Christian Aasheim, Ton Logtenberg, and Frank Larsen Preparation of Competent Cells for High-Efficiency Plasmid Transformation of Escherichia coli Yasuhiko Nakata, Xiaoren Tang, and Kazunari K. Yokoyama The Tetramethylammonium Chloride (TMAC) Method for Screening cDNA Libraries with Highly Degenerate Oligonucleotide Probes Obtained by Reverse Translation of Amino Acid Sequences Bent Honore and Peder Madsen Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides Caroline A. Austin Immunological Screening of l-Phage cDNA Expression Libraries Helen C. Hurst Cloning Sequence-Specific DNA-Binding Factors from cDNA Expression Libraries Using Oligonucleotide Binding Site Probes Ian G. Cowell Protein Interaction Cloning by Far-Western Screening of l-Phage cDNA ExpressionLibraries Shinichi Takayama and John C. Reed Yeast Two-Hybrid Library Screening Ian G. Cowell Signal Sequence Trap: Expression Cloning Method for Secreted Proteins and Type 1 Membrane Proteins Kei Tashiro, Toru Nakano, and Tasuku Honjo Isolation of Genetic Suppressor Elements (GSEs) from Random Fragment cDNA Libraries in Retroviral Vectors Andrei V. Gudkov and Igor B. Roninson Expression and Preparation of Fusion Proteins from Recombinant lgt11 Phages Sheng-He Huang and Ambrose Jong Computer Analysis of Cloned Sequences Paul R. Caron The TIGR Human cDNA Database Ewen F. Kirkness and Anthony R. Kerlavage Searching the dbEST Database Patricia Rodriguez Tome Reference cDNA Library Facilities Available from European Sources Michael P. Starkey, Yagnesh Umrania, Christopher R. Mundy, and Martin J. Bishop Index