publication . Article . Other literature type . 2021

Comparative study of autofluorescence in flat and tapered optical fibers towards application in depth-resolved fluorescence lifetime photometry in brain tissue

Marco Bianco; Antonio Balena; Marco Pisanello; Filippo Pisano; Leonardo Sileo; Barbara Spagnolo; Cinzia Montinaro; Bernardo L. Sabatini; Massimo De Vittorio; Ferruccio Pisanello;
Open Access
  • Published: 26 Jan 2021 Journal: Biomedical Optics Express, volume 12, page 993 (issn: 2156-7085, eissn: 2156-7085, Copyright policy)
  • Publisher: The Optical Society
Abstract
Abstract: As the scientific community seeks efficient optical neural interfaces with sub-cortical structures of the mouse brain, a wide set of technologies and methods is being developed to monitor cellular events through fluorescence signals generated by genetically encoded molecules. Among these technologies, tapered optical fibers (TFs) take advantage of the modal properties of narrowing waveguides to enable both depth-resolved and wide-volume light collection from scattering tissue, with minimized invasiveness with respect to standard flat fiber stubs (FFs). However, light guided in patch cords as well as in FFs and TFs can result in autofluorescence (AF) si...
Persistent Identifiers
Subjects
Medical Subject Headings: fungi
free text keywords: Atomic and Molecular Physics, and Optics, Biotechnology, Article, Optical fiber, law.invention, law, Photometry (optics), Fluorescence, Image processing, Scattering, Optics, business.industry, business, Materials science, Photon counting, Waveguide, Autofluorescence
Communities
  • FET H2020
Funded by
EC| MODEM
Project
MODEM
Multipoint Optical DEvices for Minimally invasive neural circuits interface
  • Funder: European Commission (EC)
  • Project Code: 677683
  • Funding stream: H2020 | ERC | ERC-STG
Validated by funder
,
EC| BrainBIT
Project
BrainBIT
All-optical brain-to-brain behaviour and information transfer
  • Funder: European Commission (EC)
  • Project Code: 692943
  • Funding stream: H2020 | ERC | ERC-ADG
,
EC| NanoBRIGHT
Project
NanoBRIGHT
BRInGing nano-pHoTonics into the brain
  • Funder: European Commission (EC)
  • Project Code: 828972
  • Funding stream: H2020 | RIA
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