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Microscope calibration using laser written fluorescence

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type , Preprint 08 Aug 2018Embargo end date: 01 Jan 2018 United Kingdom English Publisher:Optica Publishing GroupJournal:Optics Express, volume 26, page 21,887 (eissn: 1094-4087, Copyright policy )Funded by:UKRI | A Smart Robotic System fo..., WT | Micron Oxford: super-reso...
Authors: Alexander D. Corbett; Michael Shaw; Andrew Yacoot; Andrew Jefferson; Lothar Schermelleh; Tony Wilson; Martin Booth; +1 Authors
APC: 2,872.43 EUR

doi: 10.1364/oe.26.021887 , 10.48550/arxiv.1806.05457

pmid: 30130891

pmc: PMC6238825

arXiv: 1806.05457

Microscope calibration using laser written fluorescence

Abstract

There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and colour plane alignment using confocal and structured illumination fluorescence microscopes.

16 pages, 10 figures

Country
United Kingdom
Related Organizations
Keywords

FOS: Physical sciences, Article, Physics - Optics, Optics (physics.optics)

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    popularity
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
23
Top 10%
Top 10%
Top 10%
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