A structurally transformed lytic bacteriophage having a broad host range of Staphylococcus aureus strains and a penicillin-binding protein (PBP 2a) antibody conjugated latex beads have been used to produce a biosensor for discrimination of methicillin resistant (MRSA) and sensitive (MSSA) S. aureus strains. The lytic phages have been converted into phage spheroids and moved onto a biosensor surface by Langmuir-Blodgett technique. The produced biosensors have been tested by a quartz crystal microbalance with dissipation tracking to examine bacteria-phage interactions. Bacteria-spheroid interactions caused reduction of resonance frequency and rise in dissipation energy for both MRSA and MSSA strains. Following the bacterial binding, these sensors have been further exposed to the penicillin-binding protein antibody latex beads. Sensors analyzed with MRSA responded to PBP 2a antibody beads; although sensors tested with MSSA gave no response. This experimental distinction determines an unambiguous discrimination between methicillin resistant and sensitive S. aureus strains.