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APEX2 Proximity Proteomics Resolves Flagellum Subdomains and Identifies Flagellum Tip-Specific Proteins in Trypanosoma brucei

Authors: Daniel E. Vélez-Ramírez; Michelle M. Shimogawa; Sunayan S. Ray; Andrew Lopez; Shima Rayatpisheh; Gerasimos Langousis; Marcus Gallagher-Jones; +3 Authors

APEX2 Proximity Proteomics Resolves Flagellum Subdomains and Identifies Flagellum Tip-Specific Proteins in Trypanosoma brucei

Abstract

Sleeping sickness is a neglected tropical disease caused by the protozoan parasite Trypanosoma brucei . The disease disrupts the sleep-wake cycle, leading to coma and death if left untreated. T. brucei motility, transmission, and virulence depend on its flagellum (cilium), which consists of several different specialized subdomains.

Country
United States
Keywords

Proteomics, Trypanosoma, Proteome, Immunology, Trypanosoma brucei brucei, Protozoan Proteins, 579, Microbiology, DNA-(Apurinic or Apyrimidinic Site) Lyase, 2.2 Factors relating to the physical environment, cell signaling, Humans, Biological Sciences, Endonucleases, Multifunctional Enzymes, QR1-502, Vector-Borne Diseases, Infectious Diseases, Good Health and Well Being, Flagella, Biochemistry and Cell Biology, flagella, Infection, signaling, Research Article, Signal Transduction

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    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    15
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 10%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
15
Top 10%
Average
Top 10%
Green
gold