A monocistronic transcript for a trypanosome variant surface glycoprotein.
Copyright policy )A monocistronic transcript for a trypanosome variant surface glycoprotein.
Many protein-encoding genes of African trypanosomes are transcribed as large polycistronic pre-mRNAs that are processed into individual mRNAs containing a 5' spliced leader and 3' poly(A). The 45- to 60-kb pre-mRNAs encoding some variant surface glycoproteins (VSGs) contain as many as eight unrelated coding regions. Here we identify the promoter for a metacyclic VSG gene that is expressed without duplication in a bloodstream trypanosome clone. This 70-bp promoter is located 2 kb upstream of the telomere-linked VSG gene and directs the synthesis of a monocistronic VSG pre-mRNA lacking the 5' spliced leader. Its sequence only slightly resembles those of other known trypanosome promoters, but it does cross-hybridize with several related sequences elsewhere in the genome. These results suggest that a new class of trypanosome promoters has been found, whose function is to initiate monocistronic transcription of those VSG genes normally expressed during the metacyclic stage.
- University of Iowa United States
- Walter Reed Army Institute of Research United States
- Howard Hughes Medical Institute United States
Trypanosoma brucei rhodesiense, Base Sequence, Genes, Protozoan, Molecular Sequence Data, Restriction Mapping, Gene Expression, Structure-Activity Relationship, Gene Expression Regulation, Genes, Sequence Homology, Nucleic Acid, Mutagenesis, Site-Directed, Animals, Amino Acid Sequence, RNA, Messenger, Promoter Regions, Genetic, Sequence Alignment, RNA, Protozoan, Variant Surface Glycoproteins, Trypanosoma
Trypanosoma brucei rhodesiense, Base Sequence, Genes, Protozoan, Molecular Sequence Data, Restriction Mapping, Gene Expression, Structure-Activity Relationship, Gene Expression Regulation, Genes, Sequence Homology, Nucleic Acid, Mutagenesis, Site-Directed, Animals, Amino Acid Sequence, RNA, Messenger, Promoter Regions, Genetic, Sequence Alignment, RNA, Protozoan, Variant Surface Glycoproteins, Trypanosoma
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