publication . Article . 2012

Platelet Factor 4 and Duffy Antigen Required for Platelet Killing of Plasmodium falciparum

Brendan J. McMorran; Laura Wieczorski; Karen E. Drysdale; Jo-Anne Chan; Hong Ming Huang; Clare M. Smith; Chalachew Mitiku; James G. Beeson; Gaetan Burgio; Simon J. Foote;
Open Access
  • Published: 06 Dec 2012
  • Publisher: American Association for the Advancement of Science (AAAS)
Abstract
Platelets restrict the growth of intraerythrocytic malaria parasites by binding to parasitized cells and killing the parasite within. Here, we show that the platelet molecule platelet factor 4 (PF4 or CXCL4) and the erythrocyte Duffy-antigen receptor (Fy) are necessary for platelet-mediated killing of Plasmodium falciparum parasites. PF4 is released by platelets on contact with parasitized red cells, and the protein directly kills intraerythrocytic parasites. This function for PF4 is critically dependent on Fy, which binds PF4. Genetic disruption of Fy expression inhibits binding of PF4 to parasitized cells and concomitantly prevents parasite killing by both hum...
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free text keywords: Multidisciplinary, Virology, Platelet, Parasite hosting, Plasmodium falciparum, biology.organism_classification, biology, Recombinant DNA, law.invention, law, Antigen, Receptor, Platelet factor 4, Gene expression
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