Nucleotide Sequence of Human Preproinsulin Complementary DNA

descriptionPublicationkeyboard_double_arrow_right Article 04 Apr 1980 English Publisher:American Association for the Advancement of Science (AAAS)Journal:Science, volume 208, pages 57-59 (issn: 0036-8075, eissn: 1095-9203,

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Authors: I, Sures; D V, Goeddel; A, Gray; A, Ullrich;

doi: 10.1126/science.6927840

pmid: 6927840

Nucleotide Sequence of Human Preproinsulin Complementary DNA

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Abstract

Recombinant bacterial plasmids that contain DNA complementary to human preproinsulin messenger RNA have been constructed. One clone contains the entire preproinsulin coding region, as well as the 3′ untranslated region of the messenger RNA and eight nucleotides of the 5′ untranslated region. Additional sequence information for the 5′ untranslated region was obtained with the use of insulinoma messenger RNA in conjunction with specific primers from the cloned DNA for enzymatic chain termination sequence analysis. The results confirm the amino acid sequence of human proinsulin previously determined, and predict the amino acid sequence of the human preproinsulin signal peptide.

Related Organizations

Stanford University
United States
GENENTECH INC
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United States Department of Veterans Affairs
United States

Keywords

Base Sequence, Nucleotides, DNA, Recombinant, Nucleic Acid Hybridization, Humans, Insulin, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Protein Precursors, Proinsulin

3 Research products, page 1 of 1

Homo sapiens (human) insulin
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Homo sapiens insulin (INS) gene, complete cds.
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