Protoplasts: Preparation from Higher Plants

descriptionPublicationkeyboard_double_arrow_right Article 14 Oct 1966 English Publisher:American Association for the Advancement of Science (AAAS)Journal:Science, volume 154, pages 280-281 (issn: 0036-8075, eissn: 1095-9203,

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Authors: A W, Ruesink; K V, Thimanndagger;

doi: 10.1126/science.154.3746.280

pmid: 17810310

Protoplasts: Preparation from Higher Plants

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Abstract

Protoplasts can be readily prepared from higher-plant tissue with a concentrated Myrothecium cellulase preparation. An ionic osmoticum produced a higher yield and more viable protoplasts than did the nonionic mannitol osmoticum. Addition of polyuronidases or of ethylenediaminetetra-acetate did not increase the yield. Protoplasts could be prepared from root, leaf, and callus tissue as well as from coleoptiles.

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