In this contribution an overview of fundamentals, possibilities, and applications of acoustic levitation in the field of analytical, physical, and atmospheric chemistry investigated by Neidhart and co-workers since the 1990s will be given. Started with sample preparation procedures in acoustically levitated drops, e.g., analytical enrichment, liquid–liquid extraction, and acid-base titration [E. Welter and B. Neidhart, Fresenius J. Anal. Chem. 357, 345–350 (1997); R. Eberhard and B. Neidhart, ibid. 365, 475–479 (1999); O. Rohling, C. Weitkamp, and B. Neidhart, ibid. 368, 125–129 (2000)], the work has been continued in evaporation processes of liquids [R. Tuckermann, S. Bauerecker, and B. Neidhart, Analytical Bioanal. Chem. 372, 122–127 (2002)], crystallization, and freezing processes in acoustically levitated drops, formation and characterization of monomolecular films on the surface of levitated drops, formation and growth of particles [S. Bauerecker and B. Neidhart, J. Chem. Phys. 109(10), 3709–3712 (1998); Science 282, 2211–2212 (1998)] and trapping of heavy gases [R. Tuckermann, B. Neidhart, E. G. Lierke, and S. Bauerecker, Chem. Phys. Lett. 363, 249–354 (2002)] in stationary ultrasonic fields. Examples and results of these investigations will be presented.