publication . Article . 2019

A non-myeloablative chimeric mouse model accurately defines microglia and macrophage contribution in glioma.

Yu, K.; Youshani, A. S.; Wilkinson, F. L.; O'Leary, C.; Cook, P.; Laaniste, L.; Liao, A.; Mosses, D.; Waugh, C.; Shorrock, H.; ...
Open Access English
  • Published: 18 Feb 2019
  • Publisher: Wiley
  • Country: United Kingdom
Abstract
Resident and peripherally-derived glioma associated microglia/macrophages (GAMM) play a key role in driving tumour progression, angiogenesis, invasion, and attenuating host immune responses. Differentiating these cells' origins is challenging and current pre-clinical models such as irradiation-based adoptive transfer, parabiosis and transgenic mice have limitations. We aimed to develop a novel non-myeloablative transplantation (NMT) mouse model that permits high levels of peripheral chimerism without blood-brain barrier (BBB) damage or brain infiltration prior to tumour implantation.NMT dosing was determined in C57BL/6J or Pep3/CD45.1 mice conditioned with conce...
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free text keywords: chimeric mouse model, Glioblastoma, glioma microenvironment, macrophage, microglia, nonmyeloablative transplantation, Original Article, Original Articles, chimeric mouse model, Glioblastoma, glioma microenvironment, macrophage, microglia, nonmyeloablative transplantation, Physiology (medical), Clinical Neurology, Neurology, Histology, Pathology and Forensic Medicine, Microglia, medicine.anatomical_structure, medicine, MERTK, Haematopoiesis, Adoptive cell transfer, Cancer research, Glioma, medicine.disease, Bone marrow, Biology, Transplantation, Population, education.field_of_study, education
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Funded by
EC| INMIND
Project
INMIND
Imaging of Neuroinflammation in Neurodegenerative Diseases
  • Funder: European Commission (EC)
  • Project Code: 278850
  • Funding stream: FP7 | SP1 | HEALTH
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