AbstractAedes aegyptiL. (Diptera: Culicidae), being the primary vector of pathogenic arboviruses, is a target for the development of novel genetic approaches to complement current conventional vector control strategies such as the combined sterile insect and incompatible insect technique (SIT/IIT). A transinfected line ofAe. aegypticarrying thewAlbBWolbachiastrain (WB2) was introgressed into two genomic backgrounds, Brazil and Mexico, producing two newAe. aegyptistrains (WB2‐BRA and WB2‐MEX). These strains were evaluated with respect to several life‐history traits such as fecundity, fertility, longevity, pupa size, pupation curve, and male mating competitiveness, as well as their response to irradiation. Our results show that the impact ofWolbachiainfection depends on the genomic background and that the Brazilian one had no significant effect, whereas the Mexican one negatively affected fertility, longevity, and pupal size. Interestingly,Wolbachia‐infectedAe. aegyptilines required a lower irradiation dose to achieve complete female sterility than the uninfected ones. The present findings are discussed given the potential use ofWolbachia‐infectedAe. aegyptilines in combined SIT/IIT population suppression programs.